'Sequence saturation mutagenesis with tunable mutation frequencies'. 'Sequence Saturation Mutagenesis (SeSaM): a novel method for directed protein evolution'. A line of business for the production and sales of premium reagents for biotechnology application is under development. Besides, SeSaM-Biotech offers single enzyme services for assay development, computational analysis and in the consulting sector. These mutagenesis methods are applied to generate gene libraries for customers or are used in comprehensive protein engineering projects including random and knowledge-based diversity generation, assay development and variant screening. SeSaM-Biotech holds the patent for the Sequence Saturation Mutagenesis method for random mutagenesis, but applies also additional techniques for random and focused mutagenesis such as the licensed OmniChange method. With continuous research by SeSaM-Biotech and the Schwaneberg group, several modifications of the SeSaM method were introduced that especially allowed for the introduction of consecutive mutations and a further shift of the mutational bias towards transversion substitutions, generating a versatile tool for protein engineering and evolution.

By non-specific introduction of universal or degenerate bases at every position in the gene sequence, SeSaM overcomes the polymerase bias favoring transitory substitutions at specific positions but opens the complete gene sequence to a diverse array of amino acid exchanges. Schwaneberg and his group in order to overcome several of the major limitations encountered when working with standard mutagenesis methods based on simple error-prone PCR (epPCR) techniques. Besides, internal research is performed for the further development of the in-house techniques and the expansion of the product range beyond protein engineering service provision. Starting from SeSaM library generation, SeSaM-Biotech now applies a variety of random and focused mutagenesis methods in combination with developing and applying high-throughput screening assays for the realization of extensive protein engineering projects according to the KnowVolution strategy. Today, it is a small company in the growing Biotechnology sector providing mainly services to diverse customers from the Biotechnology industry. Ulrich Schwaneberg who had developed and improved the patented key technology Sequence Saturation Mutagenesis (SeSaM) in the preceding years, In 2012, SeSaM-Biotech moved to its new facilities at the Leibniz-Institute for Interactive Materials (DWI) in Aachen. The SeSaM-Biotech GmbH was founded in 2008 as a spin-off of Jacobs University Bremen by Prof. 2 Sequence Saturation Mutagenesis method.The tomb where it was found is thought to have belonged to King Philip II of Macedon, father of Alexander the Great. In a few special cases, larnakes appear to have been made out of precious materials, as in the 4th century BC example found at Vergina in northern Greece, of gold, with a sun motif (the source of the Vergina Sun motif) on the lid. ĭuring the later Hellenistic period, larnakes in the form of a small terracotta sarcophagus became popular, some of which were painted in similar styles to contemporary Greek vases. They were richly decorated with abstract patterns, octopuses and scenes of hunting and cult rituals. The first larnakes appeared in Minoan times during the Greek Bronze Age, when they took the form of a ceramic coffer designed to imitate a wooden chest, perhaps on the pattern of Egyptian linen chests. The golden larnax and the golden crown of Philip II of Macedon, Vergina Museum.Ī larnax (plural larnakes) is a type of small closed coffin, box or 'ash-chest' often used as a container for human remains in ancient Greece, either a body (bent on itself) or cremated ashes.

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